PC-PLC is a key extracellular enzyme secreted by pathogenic bacteria. It can hydrolyze phosphatidylcholine in the host cell membrane, leading to cell lysis and tissue necrosis. Its activity acts as a "signal light" for pathogenicity; once lit, it indicates a high pathogenic risk. PC-PLC detection is highly valuable in the following two major fields:

1. Food Safety: Guarding the "Tip-of-the-Tongue Defense Line"

Listeria monocytogenes and Listeria ivanovii (foodborne pathogens) can secrete PC-PLC, whereas harmless Listeria species like L. innocua are typically PC-PLC negative. Rapid and accurate detection of these enzyme-producing strains in food can effectively prevent food poisoning caused by pathogen contamination and avoid outbreaks of foodborne diseases.

2. Clinical Diagnostics: Aiding "Precision Medicine"

The alpha-toxin of Clostridium perfringens (which is PC-PLC) has strong hemolytic and tissue-destroying capabilities, making it the primary pathogenic factor in gas gangrene. Rapid identification of a strain's PC-PLC activity provides crucial evidence for guiding clinical medication and assessing disease progression, thereby improving the accuracy of diagnosis and treatment.

As a dedicated chromogenic substrate for PC-PLC, the detection process of X-Phos Choline is clear and reliable:

1. Enzymatic Hydrolysis Initiation: When PC-PLC or cholinesterase comes into contact with X-Phos Choline, it specifically recognizes and hydrolyzes the phosphate ester bond of the substrate, initiating the detection reaction;

2. Indoxyl Formation: The hydrolysis process releases 5-bromo-4-chloro-3-indoxyl, which is colorless and transparent, ensuring no interference with subsequent signal observation;

3. Oxidative Color Reaction: The colorless intermediate is automatically oxidized by atmospheric oxygen to form an insoluble blue dye, resulting in a stable and non-fading signal;

4.Visual Detection and Judgment: A visible blue precipitate forms around the colonies producingPC-PLC, allowing for rapid localization of enzyme activity and quantification of enzyme levels. The results are intuitive and easy to read, without the need for complex instruments;

1.Identification of Foodborne Pathogens (Listeria genus): Typical positive bacteria include Listeria monocytogenes and Listeria ivanovii, distinguishing them from harmless Listeria (such as L. innocua), thereby ensuring food safety.

2.Virulence Determination of Clinical Pathogens (Clostridium genus): Detects the alpha-toxin (i.e., PC-PLC) of Clostridium perfringens to assist in determining its hemolytic and tissue-necrotizing virulence, providing a basis for infection diagnosis.

3.Auxiliary Assessment of Bacterial Virulence (Staphylococcus aureus): Detects PC-PLC activity in some S. aureus strains. Although not a typical marker, it can serve as a supplementary indicator of virulence, enhancing the analysis of the strain's pathogenicity.

4.Biochemical Research: Quantifies PC-PLC activity, analyzes microbial metabolic pathways, and identifies microorganisms in clinical, food, and environmental samples.

✅ High Specificity: The molecular structure is specifically designed forPC-PLC, effectively avoiding cross-interference from other phosphatases or esterases, ensuring the accuracy of detection results.

✅ In Situ Coloration: The precipitated product does not diffuse, providing high-contrast visualization of PC-PLC activity locations, making results more intuitive and easier to interpret.

✅Wide Application: Can be used in multiple research fields such as enzymology, immunology, microbiology, and drug development, meeting various needs with a single reagent.

✅ High Purity and Stability: The product's purity is verified by HPLC to be ≥98%, and it undergoes a strict quality control process to ensure batch-to-batch consistency and storage stability.