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Recommended Series | Chemiluminescence Amplifier: 5-(Tetradecanoylamino)fluorescein, Making Research Signals Leap onto the "Screen"
2025/8/20 16:30:30 Browse volume(40)Scientific research is not a solo endeavor; many key discoveries are the result of the seamless cooperation of multiple technologies. 5-(Tetradecanoylamino)fluorescein is precisely such a "synergistic assistant." Although it does not emit light directly, it can make alkaline phosphatase chemiluminescent substrates (such as AMPPD) glow brighter, for a longer and more stable duration. This makes experimental results more intuitive and easier to capture, providing researchers with more stable and sensitive detection support.
I. Product Properties
Chinese Name: 5-(Tetradecanoylamino)fluorescein
English Name: 5-(Tetradecanoylamino)fluorescein
CAS No.: 75796-37-1
Molecular Formula: C34H39NO6
Molecular Weight: 557.69
Specification: 100mg/500mg/1g bottle (custom packaging available)
Appearance: Light yellow powder
Catalog No.: G05070005
5-(Tetradecanoylamino)fluorescein is a fluorescent probe with a long-chain fatty acid structure that can bind to proteins or lipids. It is an enhancer for the alkaline phosphatase system, enhancing the chemiluminescent determination of serum alkaline phosphatase and improving detection sensitivity.
II. Application Scenarios
1. Alkaline Phosphatase (ALP) Activity Detection: As an ALP chemiluminescence enhancer, it strengthens the fluorescent signal generated in the ALP-catalyzed reaction, making the chemiluminescence stronger and more durable.
2. DNA Quantification: Used in synergy with the nucleic acid dye SYBR Green I, it can bind irreversibly to DNA, enabling quantitative detection of DNA.
3. Cell Imaging: In cell imaging, the chemiluminescence enhancer can help researchers observe subtle intracellular structures and dynamic changes more clearly.
4. Protein Detection: Used for protein labeling and detection, it enhances the chemiluminescent signal, thereby increasing detection sensitivity.
III. Product Advantages
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High Sensitivity: Significantly enhances signal intensity, aiding in the identification of trace target molecules.
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High Accuracy: Stable chemiluminescent enhancement effect ensures the reproducibility of experimental data.
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Versatile Applications: Suitable for various fields such as ALP activity detection, DNA quantification, cell imaging, and protein detection.
IV. Detection Principle
5-(Tetradecanoylamino)fluorescein enhances signal performance through two key processes. First, its molecule absorbs external light energy to enter an excited state, then quickly returns to the ground state, releasing part of the energy as an enhanced chemiluminescent signal. Second, it can bind with fluorescein to form a stable complex, fully activating the originally non-luminescent fluorescein. These two mechanisms work together to significantly enhance the signal, providing reliable support for high-sensitivity detection.
Extended Services
We also offer a variety of fluorescent dyes and related raw materials. To meet different needs, we provide customized services, including fluorescent labeling solution design, composite probe custom development, and detection optimization services, precisely matching your research and application needs. Please call +86-13302967066!
For related products such as blood cell dyes and buffer salts, you can visit the [MedSun official website] to view the complete product line and technical specifications.
Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.
Official WeChat account: Shenzhen Hepattack Bioscience & Technology Co. Ltd.
Official Website: http://www.hepattack.com
Hotline: +86 0755-23251735
QQ: 2370561481
Email: sales@hepattack.com
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