Chinese Name: Anaerobic Bacteria-β-D-Galactoside Substrate

English Name: Anaerobic β-D-galactosidase substrate

Abbreviation: hpt1010

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Purity: HPLC ≥98%

Product Number: hpt1010

Stock: In stock

Color: Orange-yellow (Insoluble precipitate)

The color development mechanism of the Anaerobic Bacteria-β-D-Galactoside Substrate (hpt1010) is fundamentally different from traditional indole substrates: the core lies in intramolecular condensation reaction – color development occurs without oxygen after enzymatic cleavage. The whole process follows a three-step logic of "enzymatic cleavage-cyclization-color development":

1. Enzyme recognition and bond cleavage: β-Galactosidase specifically recognizes and hydrolyzes the β-D-galactoside bond in the substrate, releasing a colorless alditol dye precursor.

2. Intramolecular condensation: The released precursor molecule spontaneously undergoes intramolecular condensation without the need for oxygen or any auxiliary oxidant, forming a conjugated chromogenic structure.

3. Precipitation and color development: The condensation product is an insoluble orange-yellow dye that precipitates in situ on the colony, providing a concentrated signal without diffusion.

The core of this mechanism is that the color development step is completely independent of oxygen. Traditional X-Gal must undergo oxygen-dependent oxidative dimerization to turn blue after enzymatic cleavage; under anaerobic conditions, the oxidation efficiency is extremely low, resulting in weak color development or false negatives. The condensation reaction of hpt1010 yields consistent color development under both aerobic and anaerobic conditions, with a stable and reliable signal.

• Anaerobic Bacteria Detection: The Anaerobic Bacteria-β-D-Galactoside Substrate is specifically designed for the screening and identification of β-galactosidase-positive bacteria under anaerobic conditions, presenting distinct orange-yellow colonies without the need for oxygen. It is suitable for the detection of enzyme activity in obligate anaerobes such as Bacteroides and Bifidobacterium, as well as facultative anaerobes like E. coli.

• Food Microbiological Safety Testing: Suitable for samples requiring anaerobic culture, allowing direct color development and avoiding the lag in color development of traditional substrates under oxygen-limited conditions, thus improving detection efficiency.

• Environmental Water Monitoring: For coliform group detection in drinking water and surface water, compatible with both aerobic and anaerobic conditions, reducing false negatives caused by oxygen limitation and ensuring rapid detection of anaerobic bacteria in water.

✅ Anaerobic Color Development Mechanism

Does not rely on oxygen or auxiliary reagents; spontaneous condensation and color development after enzymatic cleavage, with signal intensity consistent under anaerobic and aerobic conditions.

✅ Non‑toxic Intermediates

The color development process does not generate toxic oxidative intermediates, does not inhibit the growth of sensitive anaerobic bacteria, and provides higher detection sensitivity.

✅ Vivid Signal, No Diffusion

The color development product is an insoluble orange-yellow precipitate that deposits precisely in situ on the colony without edge diffusion. It creates a clear orthogonal color difference with indole‑based blue substrates, making weak‑positive colonies less likely to be missed.

✅ Simplified Workflow

No need to add additional oxidants, diazonium salts, or other auxiliary reagents; the substrate can be used directly by adding it to the culture medium. The detection steps are streamlined, operation is faster, and toxicity is lower.

✅ Cost Reduction

Quality comparable to imported products, service rooted locally – the value of domestic substitution lies in the dual realization of cost and delivery efficiency.