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In Stock | PNP-α-NeuNAc (CAS: 26112-88-9): The Classic “Gold Standard” for Sialidase Activity Detection

2025/12/4 14:23:48 Browse volume(6)
Sialidase Chromogenic Substrate

2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid


In the fields of glycobiology, infectious disease diagnosis, and antiviral drug development, Sialidase (Sialidase / Neuraminidase) is an extremely important biomarker and drug target. Whether evaluating influenza virus drug resistance or developing rapid diagnostic kits for bacterial vaginosis (BV), choosing a substrate with high specificity and stability is crucial.

2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid (PNP-α-NeuNAc, 26112-88-9) is a classic chromogenic substrate in this field and is widely included in various enzymatic detection kits and pharmacopoeia standard methods.

I. Product Overview

26112-88-9 Structure

Chinese Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid

English Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid

Abbreviation: PNP-α-NeuNAc

CAS No.: 26112-88-9

Molecular Formula: C17H22N2O11

Molecular Weight: 430.37

Specification: 1 mg / 5 mg / 10 mg (Custom packaging available)

Appearance: Off-white powder

Item No.: G01060050

Stock: In stock

2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid is a chromogenic substrate primarily used to detect and quantify the enzymatic activity of sialidase (neuraminidase).


II. Mechanism of Action: Precise "Enzymatic Cleavage - Color Development" Reaction

The core value of PNP-α-NeuNAc lies in its ability to convert invisible enzyme activity into a visible optical signal. The reaction process is as follows:

1. Specific Recognition: The substrate structure mimics natural sialylglycosides and can be specifically recognized by sialidases from viral, bacterial, or mammalian sources.

2. Enzymatic Hydrolysis: The enzyme cleaves the α-ketosidic bond, releasing free p-Nitrophenol (pNP) and N-acetylneuraminic acid.

3. Signal Amplification: The released pNP rapidly converts into a bright yellow phenolate ion upon addition of an alkaline stop solution.

4. Quantitative Analysis: The absorbance at 405 nm is measured using a microplate reader to accurately calculate enzyme activity units.


III. Application Scenarios

1. Basic Enzymology Research: Used to study the enzymatic properties, catalytic mechanisms, and kinetic parameters of sialidases;

2. Inhibitor Screening and Drug Development: As a standard substrate, it can be used for high-throughput screening of sialidase inhibitors targeting influenza viruses, bacteria, and other diseases, and to evaluate their efficacy;

3. Pathogen Detection: Used to detect neuraminidase activity from bacteria and viruses in clinical or environmental samples, serving pathogen diagnosis and microbiological research;


IV. Core Advantages

✅ High Purity: HPLC purity control ensures low free pNP background, providing a high signal-to-noise ratio for sensitive detection of even trace enzyme activity;

✅ High Specificity: Specifically recognizes and responds to sialidase with low non-specific background, ensuring reliable detection results;

✅ Batch-to-Batch Consistency: Strict synthesis process control ensures consistent colorimetric response between different batches, suitable for industrial kit production;

✅ Good Water Solubility: Easy to prepare high-concentration stock solutions, meeting the needs of high-throughput screening (HTS);

✅ Direct Supply from Stock: Independently developed and produced by Hepattack Bioscience & Technology, ensuring supply chain security from the source, guaranteeing project continuity, with competitive pricing;


V. Related Product Recommendations

1. X-NeuNAc Sodium Salt

160369-85-7 Structure

Abbreviation: X-NeuNAc Sodium Salt

Chinese Name: 5-Bromo-4-chloro-3-indolyl N-acetyl-α-D-neuraminic acid sodium salt

English Name: 5-Bromo-4-chloro-3-indolyl N-acetyl-α-D-neuraminic acid sodium

CAS No.: 160369-85-7

Molecular Formula: C19H21BrCIN2NaO9

Molecular Weight: 559.72

Item No.: G01010008


2. PNP-α-NeuNAc Ammonium Salt

210418-02-3 Structure

Abbreviation: PNP-α-NeuNAc Ammonium Salt

Chinese Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid ammonium salt

English Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid ammonium salt

CAS No.: 210418-02-3

Molecular Formula: C17H25N3O11

Molecular Weight: 447.40

Item No.: G01060048


3. PNP-α-NeuNAc Sodium Salt

123549-14-4 Structure

Abbreviation: PNP-α-NeuNAc Sodium Salt

Chinese Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid sodium salt

English Name: 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic acid sodium salt

CAS No.: 123549-14-4

Molecular Formula: C17H21N2NaO11

Molecular Weight: 452.35

Item No.: G01060049

Choose PNP-α-NeuNAc to ensure "standard-grade" accuracy in every sialidase assay.

For related chromogenic substrates or custom packaging services, please contact +86 13302967066!

Hepattack Bioscience & Technology is dedicated to providing reliable domestic alternatives for gene sequencing, molecular diagnostics, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can browse the complete product line and technical specifications on the 【MedSun Biotechnology Website】.


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