Imagine: a small molecule that does not emit light itself is instantly "lit up" when it encounters a specific protease—this is the brilliance of the fluorescent substrate method.

1. Dormant State

In the initial state, the fluorophore AMC in the H-Leu-AMC · HCl molecule is "locked" by leucine, its fluorescence signal is quenched, and the entire system remains in an extremely low background "silent" state.

2. Precise Recognition

When the target protease, such as leucine aminopeptidase (LAP), appears in the system, it acts like a custom key, precisely recognizing and cleaving the amide bond between leucine and AMC.

3. Signal Activation

Once the "lock" is opened, free AMC molecules are instantly released and emit strong, stable blue fluorescence under specific excitation light.

This fluorescence signal, emerging from nothing, has an intensity that is strictly linearly related to enzyme activity, thus transforming the invisible enzymatic reaction into clear, quantifiable "highlight" data.

Chinese Name: L-Leucyl-7-amido-4-methylcoumarin hydrochloride

English Name: L-Leucine-7-amido-4-methylcoumarin hydrochloride

Abbreviation: H-Leu-AMC · HCl

CAS No.: 62480-44-8

Molecular Formula: C16H21ClN2O3

Molecular Weight: 324.81

Specification: 100 mg / 1 g / 10 g (Custom packaging supported)

Form: Off-white powder

Item No.: G04050016

Transport: Low-temperature transport

H-Leu-AMC · HCl is a commonly used fluorescent substrate widely used for the activity detection of proteases, aminopeptidases, and related hydrolases. The molecule consists of L-leucine linked to 4-methylcoumarin via an amide bond. When hydrolyzed by specific enzymes, it releases the luminescent molecule AMC, enabling real-time, sensitive fluorescence detection.

1. Leucine Aminopeptidase Activity Analysis: Precisely detect the function of this enzyme in cell metabolism and disease development, providing reliable data for mechanism research.

2. Cell Biology Research: Suitable for real-time monitoring of related protease activity in processes such as cell apoptosis and signal transduction pathways.

3. In Vitro Diagnostic Raw Material Development: Used to construct diagnostic kits for detecting enzyme levels in serum or tissues, aiding in the auxiliary diagnosis of diseases such as liver disease and tumors.

✅ Excellent Signal: Optimized molecular structure ensures efficient enzymatic cleavage and rapid signal release. Wide linear range can sensitively capture enzyme activity changes from weak to strong.

✅ Precise Structure: Raw material production undergoes strict structural confirmation by NMR (HPLC), ensuring absolute correctness of the molecular structure and guaranteeing the specificity of the enzymatic reaction from the source.

✅ Batch-to-Batch Consistency: Strict quality control system ensures excellent batch consistency. No need to repeatedly optimize experimental conditions when changing batches, ensuring project continuity.

✅ Worry-Free Supply: Relying on localized large-scale production capacity, we provide stable supply from milligram to kilogram levels. Say goodbye to long import lead times and supply disruption risks, ensuring your supply chain security.