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Recommended Series | U-3ʹ-BCIP: Clearer Signals, Easier Detection—Specifically Designed for RNase and Phosphatase Assays

2025/8/29 15:20:03 Browse volume(244)


RNase Chromogenic Substrate

U-3ʹ-BCIP


In an era of molecular diagnostics pursuing ultimate sensitivity, background noise is a hidden cost.

Amplifying weak RNase A events into clear, precipitating signals is becoming a common language from R&D to mass production.

The real solution starts at the source—choosing a chromogenic substrate that can thoroughly reduce background and significantly improve the signal-to-noise ratio.

I. Working Principle

U-3ʹ-BCIP is a high-sensitivity chromogenic substrate specifically designed for detecting the activity of Ribonuclease A (RNase A). Its mechanism is based on enzyme-specific hydrolysis: under the specific catalysis of RNase A, the phosphodiester bond in the substrate molecule is hydrolyzed, releasing an indole group. This group undergoes oxidative dimerization to form an insoluble blue/purple precipitate, accurately localizing the enzyme's active site.

The value of this mechanism, based on enzymatic reaction and colorimetric output, extends far beyond theory:

  • High Signal-to-Noise Ratio: The precipitating signal does not easily diffuse, resulting in extremely low background.

  • Strong Stability: The colored result is long-lasting, facilitating recording and analysis.

  • Broad Compatibility: Suitable for both solid-phase and liquid-phase detection systems.


II. Application Scenarios

1. Food Safety Monitoring: Acts as a high-sensitivity "hygiene indicator" to assess food cleanliness, contamination risk, and quality stability, providing early warnings for foodborne risks.

2. Clinical Adjuvant Diagnosis: Detects RNase A activity in body fluids, providing a reference for the diagnosis and prognostic assessment of diseases such as pancreatic function and malignant tumors.

3.  RNase A Activity Assay: Used for quality assessment of recombinant RNase A and purity testing of enzyme preparations, featuring high sensitivity and rapid color development.


III. Product Overview

Structure of 132900-87-9

Chinese Name: Uridine-3'-(5-bromo-4-chloro-3-indolyl)-phosphate tetrabutylammonium salt

English Name: 5-Bromo-4-chloro-3-indolyl-uridine-3ʹ-phosphate tetrabutylammonium salt

Abbreviation: U-3ʹ-BCIP

CAS No.: 132900-87-9

Molecular Formula: C17H15BrClN3O9P · C16H36N

Molecular Weight: 794.12

Specification: 100 mg / 500 mg / 1 g (Custom packaging available)

Appearance: Off-white lyophilized powder

Catalog No.: G07020001


IV. Core Advantages

High Specificity: Structure optimized for RNase A recognition, reducing non-specific hydrolysis and eliminating "false positive" interference.

High Contrast: Lyophilization process plus molecular modification significantly reduces the rate of spontaneous hydrolysis, resulting in clean color development and easier result interpretation.

Precise Localization: Generates an insoluble precipitate for accurate signal localization, particularly suitable for in-situ detection and colony screening.

Reliable Supply: In-house domestic production system breaks import restrictions, supports flexible customization, and meets the needs of the entire chain from R&D to mass production.



Extended Services

Accurate enzyme activity detection originates from a reliable substrate choice.

With its excellent signal-to-noise ratio and stability, U-3ʹ-BCIP helps you achieve clearer and more credible experimental results.

For related nuclease substrates or custom services, please call +86-13302967066!

For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the [MedSun official website].

In Vitro Molecular Diagnostics | Gene Sequencing | Microbial Detection


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Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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